Ukunqunywa Kokuqukethwe kwe-Oxalic Acid Ezithelweni Nasemifinweni Nge-High-Performance Liquid Chromatography (HPLC)
1. Isimiso Sokuhlola kanye Nomklamo Wendlela
Njenge-asidi evamile ye-organic ezithelweni nasemifino, okuqukethwe yi-oxalic acid kuthinta ngqo ukunambitheka kanye nenani lokudla okunomsoco kokudla. Lokhu kuhlola kusebenzisa i-reversed-phase high-performance liquid chromatography (RP-HPLC). Ngaphansi kwezimo ze-acidic mobile phase, i-oxalic acid ihlukaniswe yi-baseline nezinto eziphazamisayo kusetshenziswa ikholomu ye-C18 chromatographic. Isethi ye-ultraviolet detector ku-210 nm isetshenziselwa ukuhlaziya inani, ngokusekelwe ezicini zokumuncwa kwe-UV kwamaqembu e-carboxyl kuma-molecule e-oxalic acid.
2. Ijika Elijwayelekile kanye Nokulungiselela Isampula
Indlela yokuxuba i-gradient isetshenziswa ukulungiselela izixazululo ezijwayelekile: linganisa ngokunembile u-25.0 mg we-oxalic acid dihydrate bese uxuba ku-25 mL ngamanzi ahlanzekile kakhulu ukuze uthole isixazululo sesitoko esingu-1 mg/mL. Lokhu kuxuba ngokulandelana ochungechungeni lwezixazululo ezijwayelekile ku-50, 100, 200, 400, kanye no-800 µg/mL, lapho ukuhlushwa ngakunye kufakwe ku-triplicate.
Ukulungiselela isampula, kusetshenziswa ukukhishwa okusizwa yi-microwave: linganisa u-5.00 g we-homogenate yezithelo/yemifino, engeza u-10 mL wesisombululo se-hydrochloric acid esingu-0.1 mol/L, uphathe ku-60°C nge-microwave imizuzu eyi-10, uhlunge ngesihlungi se-membrane esingu-0.45 µm, bese uqoqa i-filtrate ukuze ihlolwe.
3. Ukuthuthukisa Izimo ze-Chromatographic
Isigaba esihambayo siyisistimu ye-potassium dihydrogen phosphate buffer (pH 2.5)–acetonitrile (95:5), enezinga lokugeleza elingu-0.8 mL/min kanye nokushisa kwekholomu okungu-35°C. Ukulungisa isilinganiso se-acetonitrile kukhombisa ukuthi lapho isigaba se-organic sidlula u-10%, isikhathi sokugcina se-oxalic acid sincipha sibe ngaphansi kwemizuzu emi-3, kodwa kwenzeka umsila ophezulu. Ngaphansi kwezimo zokugcina ezilungiselelwe kahle, isikhathi sokugcina se-oxalic acid singamaminithi angu-4.2, sifinyelela ukuhlukaniswa okuphelele ne-citric acid eseduze kanye ne-malic acid (isixazululo > 1.5).
4. Ukuqinisekiswa Kwendlela
Ukuqinisekiswa kobubanzi obuqondile kukhombisa ubudlelwano obuhle obuqondile phakathi kwendawo ephakeme kanye nokuhlushwa okuphakathi kuka-10–1000 µg/mL (R² = 0.9993). Umkhawulo wokuthola (i-LOD), onqunywa yindlela yesilinganiso sesignali kuya komsindo, ngu-0.5 µg/mL. Ekuhlolweni kokuphindaphinda, i-RSD yezinqumo eziyisithupha eziphindaphindwayo zesampula yesipinashi efanayo ingu-1.8%. Ukuhlolwa kokubuyisa i-spike emazingeni amathathu (80%, 100%, kanye no-120%) kuveza isilinganiso sokubuyisa esingu-98.2%, 102.4%, kanye no-97.8%, ngokulandelana, kuhlangabezana nezidingo zokuhlaziywa kwenani.
5. Ukuhlaziywa Kwamasampula Angempela
Izithelo nemifino eziyisithupha ezitholakala emakethe zahlolwa: isipinashi (356 ± 12 mg/100 g), i-celery (215 ± 9 mg/100 g), utamatisi (18 ± 2 mg/100 g), i-apula (6 ± 1 mg/100 g), ubhanana (awutholakalanga), kanye ne-broccoli (89 ± 5 mg/100 g). Ukuqhathaniswa nendlela ejwayelekile kazwelonke (GB 5009.277-2016) kukhombisa ukuthi ukuphambuka okuhlobene phakathi kwalezi zindlela ezimbili kungaphansi kuka-5%, okuqinisekisa ukuthembeka kwale ndlela. Ikakhulukazi, indlela ye-HPLC ibonisa ukuzwela okuphezulu kunezindlela zendabuko ze-titration lapho kuhlaziywa amasampula okuqukethwe okuphansi.
6. Amanothi Ayisihluthulelo
Lawula ngokuqinile inani le-pH ngesikhathi sokwelashwa kwesampula. Uma i-pH yesithako idlula u-3, imvula ye-calcium oxalate izoholela emiphumeleni yokuhlolwa ephansi. Isigaba esihambayo kufanele silungiswe sisha nsuku zonke ukuze kugwenywe imvula kasawoti kanye nokuvaleka kwekholomu. Ngemva kwemijovo engu-20, hlanza ikholomu ngamanzi e-acetonitrile (30:70) imizuzu engu-30 ukuvimbela ukonakala kokusebenza kahle kwekholomu. Kumasampula aqukethe umbala (isb. iklabishi elinsomi), kunconywa ukwengeza isinyathelo sokuhlanza ukukhishwa kwesigaba esiqinile ngaphambi komjovo.
7. Iziqondiso Zokusebenzisa Nokukhulisa
Le ndlela inganwetshwa ekutholeni i-oxalic acid kumasampula ebhayoloji njengomchamo negazi. Ngokulungisa ukwakheka kwesigaba esihambayo (isb., ukwengeza i-tetrabutylammonium hydroxide), i-oxalic acid kanye nama-metabolites ayo kunganqunywa ngasikhathi sinye. Kuhlanganiswe ne-mass spectrometry detector, kungasungulwa indlela yokuthola umkhondo enembile kakhulu. Embonini yokucubungula ukudla, le ndlela inganikeza ukwesekwa kwedatha ebalulekile ekukhetheni izinhlobo ze-low-oxalic acid kanye nokwenza ngcono izinqubo zokupheka.
I-Zibo Anhao Chemical Co., Ltd. – I-Oxalic Acid
I-Zibo Anhao Chemical Co., Ltd. ingumhlinzeki othembekile futhi ithumela ngaphandle i-oxalic acid esezingeni eliphezulu (izinga lezimboni, 99.6% min) evela eShandong, eShandong, eShayina. I-oxalic acid yethu iyi-crystalline solid emhlophe, eyaziwa nangokuthi i-ethanedioic acid, enefomula yama-molecule i-C₂H₂O₄. Inokuhlanzeka okuphezulu, ukungcola okuphansi, kanye nekhwalithi ezinzile kuzo zonke izinhlobo, ihlangabezana nezidingo zezindwangu, isikhumba, i-metallurgy, ukucubungula umhlaba ongavamile, ukuhlanza amakhemikhali, kanye nezimboni zokwakha.
Sihlinzeka nge-oxalic acid engamanzi ne-dihydrate, etholakala ezikhwameni ezingama-25kg, ezikhwameni ezingama-50kg, kanye nasezikhwameni ezinkulu ezingama-1000kg. Ngezinqubo eziqinile ze-QC, imibiko ephelele yokuhlola, kanye nezinhlelo zokuphakelwa eziguquguqukayo, siqinisekisa ukulethwa okuzinzile kanye nokusebenza okuqhubekayo kwabathengi bomhlaba wonke. Imikhiqizo yethu ithunyelwe kabanzi eNingizimu-mpumalanga ye-Asia, eYurophu, e-Afrika naseMelika, ithola ukuqashelwa ngekhwalithi ethembekile kanye namanani ancintisanayo.
Khetha i-Zibo Anhao Chemical ukuze uthole ukuhlinzekwa okuthembekile kwe-oxalic acid kanye nensizakalo yamakhemikhali eyodwa yobungcweti.
Khetha i-Zibo Anhao Chemical ye-oxalic acid ehlanzekile kakhulu enikeza ukusebenza okuzinzile kanye nenani lebhizinisi lakho. Xhumana nathi ukuze uthole ama-oda amaningi noma imininingwane yobuchwepheshe: info@anhaochemical.com
